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cell signalling cat3 9165  (Cell Signaling Technology Inc)


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    Cell Signaling Technology Inc cell signalling cat3 9165
    Cell Signalling Cat3 9165, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 97/100, based on 1747 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cell+signalling+cat3+9165/beta-Actin+Rabbit+mAb/jachim_sarah_katherine__2023__cellular_senescence_circadian_rhythmicity_and_aging-471-41-41
    Average 97 stars, based on 1747 article reviews
    cell signalling cat3 9165 - by Bioz Stars, 2026-09
    97/100 stars

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    Related Articles

    Recombinant:

    Article Title: Cellular Senescence, Circadian Rhythmicity, and Aging
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Primers Integrated DNA Technologies |Cat# MM.PT.39a.22214839 30 .. Integrated DNA Technologies |Cat# Mm.PT.58.11121936 Integrated DNA Technologies |Cat# Mm.PT58.6237142 Integrated DNA Technologies |Cat# Mm.PT.58.17472803 Integrated DNA Technologies |Cat# MM.PT.58.42804808 Integrated DNA Technologies |Cat# MM.PT.58.5884610 Integrated DNA Technologies |Cat# Mm.PT58.5594166 Antibodies Cell Signalling Cat# 14020 (1:1,000) Cell Signalling Cat# 8457 (1:2,000) Cell Signalling Cat3 9165 (1:1,000) Mouse anti-rabbit IgG-HRP Santa Cruz Cat# sc-2357 (1:20,000) Rabbit anti-BMAL1 Abcam Cati# ab3350 Chemicals, peptides, and recombinant proteins ABT-263 (navitoclax) APEXBIO Cat# A3007 Cell Signalling Cati# 9803 Collagenase Type 2 Worthington Biochemical Cat# LS004176 Dexamethasone Sigma Cat# D1756 DMEM Gibco Cat# 11995-065 HEPES Buffer Gibco M-MLV Reverse Transcriptase |Invitrogen Penicillin-streptavidin- Gibco glutamine Cat# 15630-080 Cat# 28025013 Cat# 10378-016 Sigma Cati# P8340 Protein A+G Magnetic Beads {Millipore Cat# 16-663 PVDF membrane Bio-Rad Cat# 1620177 SuperSignal West Pico PLUS Thermo Fisher Scientific Cat# 34577 Chemiluminescent Substrate TRIzol reagent Invitrogen Cat# 15596026 TURBOTM DNase Thermo Fisher Scientific 0.05% Trypsin Gibco Critical commercial assays DC protein assay Bio-Rad DynabeadsTM Protein G Thermo Fisher Scientific Immunoprecipitation Kit Cat# AM2238 Cat# 25300054 Cat# 5000116 Cat# 10007D Min-Elute PCR purification kit |Qiagen Cat# 28004 NEBNext Ultra II RNA Library |NEB Cat# E7765 Prep for Illumina Nextera DNA Sample Illumina Preparation Kit NovaSeq 6000 SP Reagent Kit Illumina v1.5 (200 cycles) NovaSeq XP 2-Lane Kit v1.5 Illumina Sample Prep Kit Deposited data BMAL1-ChIP-seq GEO database Cat# FC-121-1030 Cat# 20040719 Cat# 20043130 Cat# 20020595 GEO: GSE229009 31 .. RNA-seq (Ctrl and Senescent |GEO database WT) GEO: GSE229010 GEO database RNA-seq (Ctrl and Senescent WT and Bmal1 -/-) Experimental models: Cell lines C57BL/6 WT MAFs In house GEO: GSE229011 NA Bmal1-/- MAFs Software and algorithms Prism v9.4.1 GraphPad Imagel iBright Analysis v5.1.0 Thermo Scientific In house NA https://www.graphpad.com/scientific- software/prism/ https://imagej.nih.gov/ij https://www.thermofisher.com/us/en/home /technical-resources/software- downloads/ibright-western-imager.html Incucyte Base Analysis v2022A {Sartorius GSEA v4.3.2 Broad Institute Factoextra v1.0.7 CRAN https://www.sartorius.com/en/products/live -cell-imaging-analysis/live-cell-analysis- software/incucyte-base-software https://www.gsea-msigdb.org/gsea/index.jsp https://cran.r- project.org/web/packages/factoextra/index. html Pheatmap v1.0.12 CRAN edgeR v3.36.0 162 Trimmomatic v.0.36 156 STAR aligner 157 Subread v.1.5.2 158 159 160 161 163 Burrows-Wheeler Aligner 164 v0.5.9 Picard MarkDuplicates v1.67 165 Model-Based Analysis of ChIP- |[166 seq Bedtools v2.16.2 167 https://cran.r- project.org/web/packages/pheatmap/pheat map.pdf Diffbind v2.14.0 168 MetaCycle v1.2.0 169 32

    Reverse Transcription:

    Article Title: Cellular Senescence, Circadian Rhythmicity, and Aging
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Primers Integrated DNA Technologies |Cat# MM.PT.39a.22214839 30 .. Integrated DNA Technologies |Cat# Mm.PT.58.11121936 Integrated DNA Technologies |Cat# Mm.PT58.6237142 Integrated DNA Technologies |Cat# Mm.PT.58.17472803 Integrated DNA Technologies |Cat# MM.PT.58.42804808 Integrated DNA Technologies |Cat# MM.PT.58.5884610 Integrated DNA Technologies |Cat# Mm.PT58.5594166 Antibodies Cell Signalling Cat# 14020 (1:1,000) Cell Signalling Cat# 8457 (1:2,000) Cell Signalling Cat3 9165 (1:1,000) Mouse anti-rabbit IgG-HRP Santa Cruz Cat# sc-2357 (1:20,000) Rabbit anti-BMAL1 Abcam Cati# ab3350 Chemicals, peptides, and recombinant proteins ABT-263 (navitoclax) APEXBIO Cat# A3007 Cell Signalling Cati# 9803 Collagenase Type 2 Worthington Biochemical Cat# LS004176 Dexamethasone Sigma Cat# D1756 DMEM Gibco Cat# 11995-065 HEPES Buffer Gibco M-MLV Reverse Transcriptase |Invitrogen Penicillin-streptavidin- Gibco glutamine Cat# 15630-080 Cat# 28025013 Cat# 10378-016 Sigma Cati# P8340 Protein A+G Magnetic Beads {Millipore Cat# 16-663 PVDF membrane Bio-Rad Cat# 1620177 SuperSignal West Pico PLUS Thermo Fisher Scientific Cat# 34577 Chemiluminescent Substrate TRIzol reagent Invitrogen Cat# 15596026 TURBOTM DNase Thermo Fisher Scientific 0.05% Trypsin Gibco Critical commercial assays DC protein assay Bio-Rad DynabeadsTM Protein G Thermo Fisher Scientific Immunoprecipitation Kit Cat# AM2238 Cat# 25300054 Cat# 5000116 Cat# 10007D Min-Elute PCR purification kit |Qiagen Cat# 28004 NEBNext Ultra II RNA Library |NEB Cat# E7765 Prep for Illumina Nextera DNA Sample Illumina Preparation Kit NovaSeq 6000 SP Reagent Kit Illumina v1.5 (200 cycles) NovaSeq XP 2-Lane Kit v1.5 Illumina Sample Prep Kit Deposited data BMAL1-ChIP-seq GEO database Cat# FC-121-1030 Cat# 20040719 Cat# 20043130 Cat# 20020595 GEO: GSE229009 31 .. RNA-seq (Ctrl and Senescent |GEO database WT) GEO: GSE229010 GEO database RNA-seq (Ctrl and Senescent WT and Bmal1 -/-) Experimental models: Cell lines C57BL/6 WT MAFs In house GEO: GSE229011 NA Bmal1-/- MAFs Software and algorithms Prism v9.4.1 GraphPad Imagel iBright Analysis v5.1.0 Thermo Scientific In house NA https://www.graphpad.com/scientific- software/prism/ https://imagej.nih.gov/ij https://www.thermofisher.com/us/en/home /technical-resources/software- downloads/ibright-western-imager.html Incucyte Base Analysis v2022A {Sartorius GSEA v4.3.2 Broad Institute Factoextra v1.0.7 CRAN https://www.sartorius.com/en/products/live -cell-imaging-analysis/live-cell-analysis- software/incucyte-base-software https://www.gsea-msigdb.org/gsea/index.jsp https://cran.r- project.org/web/packages/factoextra/index. html Pheatmap v1.0.12 CRAN edgeR v3.36.0 162 Trimmomatic v.0.36 156 STAR aligner 157 Subread v.1.5.2 158 159 160 161 163 Burrows-Wheeler Aligner 164 v0.5.9 Picard MarkDuplicates v1.67 165 Model-Based Analysis of ChIP- |[166 seq Bedtools v2.16.2 167 https://cran.r- project.org/web/packages/pheatmap/pheat map.pdf Diffbind v2.14.0 168 MetaCycle v1.2.0 169 32

    Magnetic Beads:

    Article Title: Cellular Senescence, Circadian Rhythmicity, and Aging
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Primers Integrated DNA Technologies |Cat# MM.PT.39a.22214839 30 .. Integrated DNA Technologies |Cat# Mm.PT.58.11121936 Integrated DNA Technologies |Cat# Mm.PT58.6237142 Integrated DNA Technologies |Cat# Mm.PT.58.17472803 Integrated DNA Technologies |Cat# MM.PT.58.42804808 Integrated DNA Technologies |Cat# MM.PT.58.5884610 Integrated DNA Technologies |Cat# Mm.PT58.5594166 Antibodies Cell Signalling Cat# 14020 (1:1,000) Cell Signalling Cat# 8457 (1:2,000) Cell Signalling Cat3 9165 (1:1,000) Mouse anti-rabbit IgG-HRP Santa Cruz Cat# sc-2357 (1:20,000) Rabbit anti-BMAL1 Abcam Cati# ab3350 Chemicals, peptides, and recombinant proteins ABT-263 (navitoclax) APEXBIO Cat# A3007 Cell Signalling Cati# 9803 Collagenase Type 2 Worthington Biochemical Cat# LS004176 Dexamethasone Sigma Cat# D1756 DMEM Gibco Cat# 11995-065 HEPES Buffer Gibco M-MLV Reverse Transcriptase |Invitrogen Penicillin-streptavidin- Gibco glutamine Cat# 15630-080 Cat# 28025013 Cat# 10378-016 Sigma Cati# P8340 Protein A+G Magnetic Beads {Millipore Cat# 16-663 PVDF membrane Bio-Rad Cat# 1620177 SuperSignal West Pico PLUS Thermo Fisher Scientific Cat# 34577 Chemiluminescent Substrate TRIzol reagent Invitrogen Cat# 15596026 TURBOTM DNase Thermo Fisher Scientific 0.05% Trypsin Gibco Critical commercial assays DC protein assay Bio-Rad DynabeadsTM Protein G Thermo Fisher Scientific Immunoprecipitation Kit Cat# AM2238 Cat# 25300054 Cat# 5000116 Cat# 10007D Min-Elute PCR purification kit |Qiagen Cat# 28004 NEBNext Ultra II RNA Library |NEB Cat# E7765 Prep for Illumina Nextera DNA Sample Illumina Preparation Kit NovaSeq 6000 SP Reagent Kit Illumina v1.5 (200 cycles) NovaSeq XP 2-Lane Kit v1.5 Illumina Sample Prep Kit Deposited data BMAL1-ChIP-seq GEO database Cat# FC-121-1030 Cat# 20040719 Cat# 20043130 Cat# 20020595 GEO: GSE229009 31 .. RNA-seq (Ctrl and Senescent |GEO database WT) GEO: GSE229010 GEO database RNA-seq (Ctrl and Senescent WT and Bmal1 -/-) Experimental models: Cell lines C57BL/6 WT MAFs In house GEO: GSE229011 NA Bmal1-/- MAFs Software and algorithms Prism v9.4.1 GraphPad Imagel iBright Analysis v5.1.0 Thermo Scientific In house NA https://www.graphpad.com/scientific- software/prism/ https://imagej.nih.gov/ij https://www.thermofisher.com/us/en/home /technical-resources/software- downloads/ibright-western-imager.html Incucyte Base Analysis v2022A {Sartorius GSEA v4.3.2 Broad Institute Factoextra v1.0.7 CRAN https://www.sartorius.com/en/products/live -cell-imaging-analysis/live-cell-analysis- software/incucyte-base-software https://www.gsea-msigdb.org/gsea/index.jsp https://cran.r- project.org/web/packages/factoextra/index. html Pheatmap v1.0.12 CRAN edgeR v3.36.0 162 Trimmomatic v.0.36 156 STAR aligner 157 Subread v.1.5.2 158 159 160 161 163 Burrows-Wheeler Aligner 164 v0.5.9 Picard MarkDuplicates v1.67 165 Model-Based Analysis of ChIP- |[166 seq Bedtools v2.16.2 167 https://cran.r- project.org/web/packages/pheatmap/pheat map.pdf Diffbind v2.14.0 168 MetaCycle v1.2.0 169 32

    Membrane:

    Article Title: Cellular Senescence, Circadian Rhythmicity, and Aging
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Primers Integrated DNA Technologies |Cat# MM.PT.39a.22214839 30 .. Integrated DNA Technologies |Cat# Mm.PT.58.11121936 Integrated DNA Technologies |Cat# Mm.PT58.6237142 Integrated DNA Technologies |Cat# Mm.PT.58.17472803 Integrated DNA Technologies |Cat# MM.PT.58.42804808 Integrated DNA Technologies |Cat# MM.PT.58.5884610 Integrated DNA Technologies |Cat# Mm.PT58.5594166 Antibodies Cell Signalling Cat# 14020 (1:1,000) Cell Signalling Cat# 8457 (1:2,000) Cell Signalling Cat3 9165 (1:1,000) Mouse anti-rabbit IgG-HRP Santa Cruz Cat# sc-2357 (1:20,000) Rabbit anti-BMAL1 Abcam Cati# ab3350 Chemicals, peptides, and recombinant proteins ABT-263 (navitoclax) APEXBIO Cat# A3007 Cell Signalling Cati# 9803 Collagenase Type 2 Worthington Biochemical Cat# LS004176 Dexamethasone Sigma Cat# D1756 DMEM Gibco Cat# 11995-065 HEPES Buffer Gibco M-MLV Reverse Transcriptase |Invitrogen Penicillin-streptavidin- Gibco glutamine Cat# 15630-080 Cat# 28025013 Cat# 10378-016 Sigma Cati# P8340 Protein A+G Magnetic Beads {Millipore Cat# 16-663 PVDF membrane Bio-Rad Cat# 1620177 SuperSignal West Pico PLUS Thermo Fisher Scientific Cat# 34577 Chemiluminescent Substrate TRIzol reagent Invitrogen Cat# 15596026 TURBOTM DNase Thermo Fisher Scientific 0.05% Trypsin Gibco Critical commercial assays DC protein assay Bio-Rad DynabeadsTM Protein G Thermo Fisher Scientific Immunoprecipitation Kit Cat# AM2238 Cat# 25300054 Cat# 5000116 Cat# 10007D Min-Elute PCR purification kit |Qiagen Cat# 28004 NEBNext Ultra II RNA Library |NEB Cat# E7765 Prep for Illumina Nextera DNA Sample Illumina Preparation Kit NovaSeq 6000 SP Reagent Kit Illumina v1.5 (200 cycles) NovaSeq XP 2-Lane Kit v1.5 Illumina Sample Prep Kit Deposited data BMAL1-ChIP-seq GEO database Cat# FC-121-1030 Cat# 20040719 Cat# 20043130 Cat# 20020595 GEO: GSE229009 31 .. RNA-seq (Ctrl and Senescent |GEO database WT) GEO: GSE229010 GEO database RNA-seq (Ctrl and Senescent WT and Bmal1 -/-) Experimental models: Cell lines C57BL/6 WT MAFs In house GEO: GSE229011 NA Bmal1-/- MAFs Software and algorithms Prism v9.4.1 GraphPad Imagel iBright Analysis v5.1.0 Thermo Scientific In house NA https://www.graphpad.com/scientific- software/prism/ https://imagej.nih.gov/ij https://www.thermofisher.com/us/en/home /technical-resources/software- downloads/ibright-western-imager.html Incucyte Base Analysis v2022A {Sartorius GSEA v4.3.2 Broad Institute Factoextra v1.0.7 CRAN https://www.sartorius.com/en/products/live -cell-imaging-analysis/live-cell-analysis- software/incucyte-base-software https://www.gsea-msigdb.org/gsea/index.jsp https://cran.r- project.org/web/packages/factoextra/index. html Pheatmap v1.0.12 CRAN edgeR v3.36.0 162 Trimmomatic v.0.36 156 STAR aligner 157 Subread v.1.5.2 158 159 160 161 163 Burrows-Wheeler Aligner 164 v0.5.9 Picard MarkDuplicates v1.67 165 Model-Based Analysis of ChIP- |[166 seq Bedtools v2.16.2 167 https://cran.r- project.org/web/packages/pheatmap/pheat map.pdf Diffbind v2.14.0 168 MetaCycle v1.2.0 169 32

    DC Protein Assay:

    Article Title: Cellular Senescence, Circadian Rhythmicity, and Aging
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Primers Integrated DNA Technologies |Cat# MM.PT.39a.22214839 30 .. Integrated DNA Technologies |Cat# Mm.PT.58.11121936 Integrated DNA Technologies |Cat# Mm.PT58.6237142 Integrated DNA Technologies |Cat# Mm.PT.58.17472803 Integrated DNA Technologies |Cat# MM.PT.58.42804808 Integrated DNA Technologies |Cat# MM.PT.58.5884610 Integrated DNA Technologies |Cat# Mm.PT58.5594166 Antibodies Cell Signalling Cat# 14020 (1:1,000) Cell Signalling Cat# 8457 (1:2,000) Cell Signalling Cat3 9165 (1:1,000) Mouse anti-rabbit IgG-HRP Santa Cruz Cat# sc-2357 (1:20,000) Rabbit anti-BMAL1 Abcam Cati# ab3350 Chemicals, peptides, and recombinant proteins ABT-263 (navitoclax) APEXBIO Cat# A3007 Cell Signalling Cati# 9803 Collagenase Type 2 Worthington Biochemical Cat# LS004176 Dexamethasone Sigma Cat# D1756 DMEM Gibco Cat# 11995-065 HEPES Buffer Gibco M-MLV Reverse Transcriptase |Invitrogen Penicillin-streptavidin- Gibco glutamine Cat# 15630-080 Cat# 28025013 Cat# 10378-016 Sigma Cati# P8340 Protein A+G Magnetic Beads {Millipore Cat# 16-663 PVDF membrane Bio-Rad Cat# 1620177 SuperSignal West Pico PLUS Thermo Fisher Scientific Cat# 34577 Chemiluminescent Substrate TRIzol reagent Invitrogen Cat# 15596026 TURBOTM DNase Thermo Fisher Scientific 0.05% Trypsin Gibco Critical commercial assays DC protein assay Bio-Rad DynabeadsTM Protein G Thermo Fisher Scientific Immunoprecipitation Kit Cat# AM2238 Cat# 25300054 Cat# 5000116 Cat# 10007D Min-Elute PCR purification kit |Qiagen Cat# 28004 NEBNext Ultra II RNA Library |NEB Cat# E7765 Prep for Illumina Nextera DNA Sample Illumina Preparation Kit NovaSeq 6000 SP Reagent Kit Illumina v1.5 (200 cycles) NovaSeq XP 2-Lane Kit v1.5 Illumina Sample Prep Kit Deposited data BMAL1-ChIP-seq GEO database Cat# FC-121-1030 Cat# 20040719 Cat# 20043130 Cat# 20020595 GEO: GSE229009 31 .. RNA-seq (Ctrl and Senescent |GEO database WT) GEO: GSE229010 GEO database RNA-seq (Ctrl and Senescent WT and Bmal1 -/-) Experimental models: Cell lines C57BL/6 WT MAFs In house GEO: GSE229011 NA Bmal1-/- MAFs Software and algorithms Prism v9.4.1 GraphPad Imagel iBright Analysis v5.1.0 Thermo Scientific In house NA https://www.graphpad.com/scientific- software/prism/ https://imagej.nih.gov/ij https://www.thermofisher.com/us/en/home /technical-resources/software- downloads/ibright-western-imager.html Incucyte Base Analysis v2022A {Sartorius GSEA v4.3.2 Broad Institute Factoextra v1.0.7 CRAN https://www.sartorius.com/en/products/live -cell-imaging-analysis/live-cell-analysis- software/incucyte-base-software https://www.gsea-msigdb.org/gsea/index.jsp https://cran.r- project.org/web/packages/factoextra/index. html Pheatmap v1.0.12 CRAN edgeR v3.36.0 162 Trimmomatic v.0.36 156 STAR aligner 157 Subread v.1.5.2 158 159 160 161 163 Burrows-Wheeler Aligner 164 v0.5.9 Picard MarkDuplicates v1.67 165 Model-Based Analysis of ChIP- |[166 seq Bedtools v2.16.2 167 https://cran.r- project.org/web/packages/pheatmap/pheat map.pdf Diffbind v2.14.0 168 MetaCycle v1.2.0 169 32

    Immunoprecipitation:

    Article Title: Cellular Senescence, Circadian Rhythmicity, and Aging
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Primers Integrated DNA Technologies |Cat# MM.PT.39a.22214839 30 .. Integrated DNA Technologies |Cat# Mm.PT.58.11121936 Integrated DNA Technologies |Cat# Mm.PT58.6237142 Integrated DNA Technologies |Cat# Mm.PT.58.17472803 Integrated DNA Technologies |Cat# MM.PT.58.42804808 Integrated DNA Technologies |Cat# MM.PT.58.5884610 Integrated DNA Technologies |Cat# Mm.PT58.5594166 Antibodies Cell Signalling Cat# 14020 (1:1,000) Cell Signalling Cat# 8457 (1:2,000) Cell Signalling Cat3 9165 (1:1,000) Mouse anti-rabbit IgG-HRP Santa Cruz Cat# sc-2357 (1:20,000) Rabbit anti-BMAL1 Abcam Cati# ab3350 Chemicals, peptides, and recombinant proteins ABT-263 (navitoclax) APEXBIO Cat# A3007 Cell Signalling Cati# 9803 Collagenase Type 2 Worthington Biochemical Cat# LS004176 Dexamethasone Sigma Cat# D1756 DMEM Gibco Cat# 11995-065 HEPES Buffer Gibco M-MLV Reverse Transcriptase |Invitrogen Penicillin-streptavidin- Gibco glutamine Cat# 15630-080 Cat# 28025013 Cat# 10378-016 Sigma Cati# P8340 Protein A+G Magnetic Beads {Millipore Cat# 16-663 PVDF membrane Bio-Rad Cat# 1620177 SuperSignal West Pico PLUS Thermo Fisher Scientific Cat# 34577 Chemiluminescent Substrate TRIzol reagent Invitrogen Cat# 15596026 TURBOTM DNase Thermo Fisher Scientific 0.05% Trypsin Gibco Critical commercial assays DC protein assay Bio-Rad DynabeadsTM Protein G Thermo Fisher Scientific Immunoprecipitation Kit Cat# AM2238 Cat# 25300054 Cat# 5000116 Cat# 10007D Min-Elute PCR purification kit |Qiagen Cat# 28004 NEBNext Ultra II RNA Library |NEB Cat# E7765 Prep for Illumina Nextera DNA Sample Illumina Preparation Kit NovaSeq 6000 SP Reagent Kit Illumina v1.5 (200 cycles) NovaSeq XP 2-Lane Kit v1.5 Illumina Sample Prep Kit Deposited data BMAL1-ChIP-seq GEO database Cat# FC-121-1030 Cat# 20040719 Cat# 20043130 Cat# 20020595 GEO: GSE229009 31 .. RNA-seq (Ctrl and Senescent |GEO database WT) GEO: GSE229010 GEO database RNA-seq (Ctrl and Senescent WT and Bmal1 -/-) Experimental models: Cell lines C57BL/6 WT MAFs In house GEO: GSE229011 NA Bmal1-/- MAFs Software and algorithms Prism v9.4.1 GraphPad Imagel iBright Analysis v5.1.0 Thermo Scientific In house NA https://www.graphpad.com/scientific- software/prism/ https://imagej.nih.gov/ij https://www.thermofisher.com/us/en/home /technical-resources/software- downloads/ibright-western-imager.html Incucyte Base Analysis v2022A {Sartorius GSEA v4.3.2 Broad Institute Factoextra v1.0.7 CRAN https://www.sartorius.com/en/products/live -cell-imaging-analysis/live-cell-analysis- software/incucyte-base-software https://www.gsea-msigdb.org/gsea/index.jsp https://cran.r- project.org/web/packages/factoextra/index. html Pheatmap v1.0.12 CRAN edgeR v3.36.0 162 Trimmomatic v.0.36 156 STAR aligner 157 Subread v.1.5.2 158 159 160 161 163 Burrows-Wheeler Aligner 164 v0.5.9 Picard MarkDuplicates v1.67 165 Model-Based Analysis of ChIP- |[166 seq Bedtools v2.16.2 167 https://cran.r- project.org/web/packages/pheatmap/pheat map.pdf Diffbind v2.14.0 168 MetaCycle v1.2.0 169 32

    Polymerase Chain Reaction:

    Article Title: Cellular Senescence, Circadian Rhythmicity, and Aging
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Primers Integrated DNA Technologies |Cat# MM.PT.39a.22214839 30 .. Integrated DNA Technologies |Cat# Mm.PT.58.11121936 Integrated DNA Technologies |Cat# Mm.PT58.6237142 Integrated DNA Technologies |Cat# Mm.PT.58.17472803 Integrated DNA Technologies |Cat# MM.PT.58.42804808 Integrated DNA Technologies |Cat# MM.PT.58.5884610 Integrated DNA Technologies |Cat# Mm.PT58.5594166 Antibodies Cell Signalling Cat# 14020 (1:1,000) Cell Signalling Cat# 8457 (1:2,000) Cell Signalling Cat3 9165 (1:1,000) Mouse anti-rabbit IgG-HRP Santa Cruz Cat# sc-2357 (1:20,000) Rabbit anti-BMAL1 Abcam Cati# ab3350 Chemicals, peptides, and recombinant proteins ABT-263 (navitoclax) APEXBIO Cat# A3007 Cell Signalling Cati# 9803 Collagenase Type 2 Worthington Biochemical Cat# LS004176 Dexamethasone Sigma Cat# D1756 DMEM Gibco Cat# 11995-065 HEPES Buffer Gibco M-MLV Reverse Transcriptase |Invitrogen Penicillin-streptavidin- Gibco glutamine Cat# 15630-080 Cat# 28025013 Cat# 10378-016 Sigma Cati# P8340 Protein A+G Magnetic Beads {Millipore Cat# 16-663 PVDF membrane Bio-Rad Cat# 1620177 SuperSignal West Pico PLUS Thermo Fisher Scientific Cat# 34577 Chemiluminescent Substrate TRIzol reagent Invitrogen Cat# 15596026 TURBOTM DNase Thermo Fisher Scientific 0.05% Trypsin Gibco Critical commercial assays DC protein assay Bio-Rad DynabeadsTM Protein G Thermo Fisher Scientific Immunoprecipitation Kit Cat# AM2238 Cat# 25300054 Cat# 5000116 Cat# 10007D Min-Elute PCR purification kit |Qiagen Cat# 28004 NEBNext Ultra II RNA Library |NEB Cat# E7765 Prep for Illumina Nextera DNA Sample Illumina Preparation Kit NovaSeq 6000 SP Reagent Kit Illumina v1.5 (200 cycles) NovaSeq XP 2-Lane Kit v1.5 Illumina Sample Prep Kit Deposited data BMAL1-ChIP-seq GEO database Cat# FC-121-1030 Cat# 20040719 Cat# 20043130 Cat# 20020595 GEO: GSE229009 31 .. RNA-seq (Ctrl and Senescent |GEO database WT) GEO: GSE229010 GEO database RNA-seq (Ctrl and Senescent WT and Bmal1 -/-) Experimental models: Cell lines C57BL/6 WT MAFs In house GEO: GSE229011 NA Bmal1-/- MAFs Software and algorithms Prism v9.4.1 GraphPad Imagel iBright Analysis v5.1.0 Thermo Scientific In house NA https://www.graphpad.com/scientific- software/prism/ https://imagej.nih.gov/ij https://www.thermofisher.com/us/en/home /technical-resources/software- downloads/ibright-western-imager.html Incucyte Base Analysis v2022A {Sartorius GSEA v4.3.2 Broad Institute Factoextra v1.0.7 CRAN https://www.sartorius.com/en/products/live -cell-imaging-analysis/live-cell-analysis- software/incucyte-base-software https://www.gsea-msigdb.org/gsea/index.jsp https://cran.r- project.org/web/packages/factoextra/index. html Pheatmap v1.0.12 CRAN edgeR v3.36.0 162 Trimmomatic v.0.36 156 STAR aligner 157 Subread v.1.5.2 158 159 160 161 163 Burrows-Wheeler Aligner 164 v0.5.9 Picard MarkDuplicates v1.67 165 Model-Based Analysis of ChIP- |[166 seq Bedtools v2.16.2 167 https://cran.r- project.org/web/packages/pheatmap/pheat map.pdf Diffbind v2.14.0 168 MetaCycle v1.2.0 169 32

    Purification:

    Article Title: Cellular Senescence, Circadian Rhythmicity, and Aging
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Primers Integrated DNA Technologies |Cat# MM.PT.39a.22214839 30 .. Integrated DNA Technologies |Cat# Mm.PT.58.11121936 Integrated DNA Technologies |Cat# Mm.PT58.6237142 Integrated DNA Technologies |Cat# Mm.PT.58.17472803 Integrated DNA Technologies |Cat# MM.PT.58.42804808 Integrated DNA Technologies |Cat# MM.PT.58.5884610 Integrated DNA Technologies |Cat# Mm.PT58.5594166 Antibodies Cell Signalling Cat# 14020 (1:1,000) Cell Signalling Cat# 8457 (1:2,000) Cell Signalling Cat3 9165 (1:1,000) Mouse anti-rabbit IgG-HRP Santa Cruz Cat# sc-2357 (1:20,000) Rabbit anti-BMAL1 Abcam Cati# ab3350 Chemicals, peptides, and recombinant proteins ABT-263 (navitoclax) APEXBIO Cat# A3007 Cell Signalling Cati# 9803 Collagenase Type 2 Worthington Biochemical Cat# LS004176 Dexamethasone Sigma Cat# D1756 DMEM Gibco Cat# 11995-065 HEPES Buffer Gibco M-MLV Reverse Transcriptase |Invitrogen Penicillin-streptavidin- Gibco glutamine Cat# 15630-080 Cat# 28025013 Cat# 10378-016 Sigma Cati# P8340 Protein A+G Magnetic Beads {Millipore Cat# 16-663 PVDF membrane Bio-Rad Cat# 1620177 SuperSignal West Pico PLUS Thermo Fisher Scientific Cat# 34577 Chemiluminescent Substrate TRIzol reagent Invitrogen Cat# 15596026 TURBOTM DNase Thermo Fisher Scientific 0.05% Trypsin Gibco Critical commercial assays DC protein assay Bio-Rad DynabeadsTM Protein G Thermo Fisher Scientific Immunoprecipitation Kit Cat# AM2238 Cat# 25300054 Cat# 5000116 Cat# 10007D Min-Elute PCR purification kit |Qiagen Cat# 28004 NEBNext Ultra II RNA Library |NEB Cat# E7765 Prep for Illumina Nextera DNA Sample Illumina Preparation Kit NovaSeq 6000 SP Reagent Kit Illumina v1.5 (200 cycles) NovaSeq XP 2-Lane Kit v1.5 Illumina Sample Prep Kit Deposited data BMAL1-ChIP-seq GEO database Cat# FC-121-1030 Cat# 20040719 Cat# 20043130 Cat# 20020595 GEO: GSE229009 31 .. RNA-seq (Ctrl and Senescent |GEO database WT) GEO: GSE229010 GEO database RNA-seq (Ctrl and Senescent WT and Bmal1 -/-) Experimental models: Cell lines C57BL/6 WT MAFs In house GEO: GSE229011 NA Bmal1-/- MAFs Software and algorithms Prism v9.4.1 GraphPad Imagel iBright Analysis v5.1.0 Thermo Scientific In house NA https://www.graphpad.com/scientific- software/prism/ https://imagej.nih.gov/ij https://www.thermofisher.com/us/en/home /technical-resources/software- downloads/ibright-western-imager.html Incucyte Base Analysis v2022A {Sartorius GSEA v4.3.2 Broad Institute Factoextra v1.0.7 CRAN https://www.sartorius.com/en/products/live -cell-imaging-analysis/live-cell-analysis- software/incucyte-base-software https://www.gsea-msigdb.org/gsea/index.jsp https://cran.r- project.org/web/packages/factoextra/index. html Pheatmap v1.0.12 CRAN edgeR v3.36.0 162 Trimmomatic v.0.36 156 STAR aligner 157 Subread v.1.5.2 158 159 160 161 163 Burrows-Wheeler Aligner 164 v0.5.9 Picard MarkDuplicates v1.67 165 Model-Based Analysis of ChIP- |[166 seq Bedtools v2.16.2 167 https://cran.r- project.org/web/packages/pheatmap/pheat map.pdf Diffbind v2.14.0 168 MetaCycle v1.2.0 169 32

    Sample Prep:

    Article Title: Cellular Senescence, Circadian Rhythmicity, and Aging
    Article Snippet: REAGENT or RESOURCE SOURCE IDENTIFIER Primers Integrated DNA Technologies |Cat# MM.PT.39a.22214839 30 .. Integrated DNA Technologies |Cat# Mm.PT.58.11121936 Integrated DNA Technologies |Cat# Mm.PT58.6237142 Integrated DNA Technologies |Cat# Mm.PT.58.17472803 Integrated DNA Technologies |Cat# MM.PT.58.42804808 Integrated DNA Technologies |Cat# MM.PT.58.5884610 Integrated DNA Technologies |Cat# Mm.PT58.5594166 Antibodies Cell Signalling Cat# 14020 (1:1,000) Cell Signalling Cat# 8457 (1:2,000) Cell Signalling Cat3 9165 (1:1,000) Mouse anti-rabbit IgG-HRP Santa Cruz Cat# sc-2357 (1:20,000) Rabbit anti-BMAL1 Abcam Cati# ab3350 Chemicals, peptides, and recombinant proteins ABT-263 (navitoclax) APEXBIO Cat# A3007 Cell Signalling Cati# 9803 Collagenase Type 2 Worthington Biochemical Cat# LS004176 Dexamethasone Sigma Cat# D1756 DMEM Gibco Cat# 11995-065 HEPES Buffer Gibco M-MLV Reverse Transcriptase |Invitrogen Penicillin-streptavidin- Gibco glutamine Cat# 15630-080 Cat# 28025013 Cat# 10378-016 Sigma Cati# P8340 Protein A+G Magnetic Beads {Millipore Cat# 16-663 PVDF membrane Bio-Rad Cat# 1620177 SuperSignal West Pico PLUS Thermo Fisher Scientific Cat# 34577 Chemiluminescent Substrate TRIzol reagent Invitrogen Cat# 15596026 TURBOTM DNase Thermo Fisher Scientific 0.05% Trypsin Gibco Critical commercial assays DC protein assay Bio-Rad DynabeadsTM Protein G Thermo Fisher Scientific Immunoprecipitation Kit Cat# AM2238 Cat# 25300054 Cat# 5000116 Cat# 10007D Min-Elute PCR purification kit |Qiagen Cat# 28004 NEBNext Ultra II RNA Library |NEB Cat# E7765 Prep for Illumina Nextera DNA Sample Illumina Preparation Kit NovaSeq 6000 SP Reagent Kit Illumina v1.5 (200 cycles) NovaSeq XP 2-Lane Kit v1.5 Illumina Sample Prep Kit Deposited data BMAL1-ChIP-seq GEO database Cat# FC-121-1030 Cat# 20040719 Cat# 20043130 Cat# 20020595 GEO: GSE229009 31 .. RNA-seq (Ctrl and Senescent |GEO database WT) GEO: GSE229010 GEO database RNA-seq (Ctrl and Senescent WT and Bmal1 -/-) Experimental models: Cell lines C57BL/6 WT MAFs In house GEO: GSE229011 NA Bmal1-/- MAFs Software and algorithms Prism v9.4.1 GraphPad Imagel iBright Analysis v5.1.0 Thermo Scientific In house NA https://www.graphpad.com/scientific- software/prism/ https://imagej.nih.gov/ij https://www.thermofisher.com/us/en/home /technical-resources/software- downloads/ibright-western-imager.html Incucyte Base Analysis v2022A {Sartorius GSEA v4.3.2 Broad Institute Factoextra v1.0.7 CRAN https://www.sartorius.com/en/products/live -cell-imaging-analysis/live-cell-analysis- software/incucyte-base-software https://www.gsea-msigdb.org/gsea/index.jsp https://cran.r- project.org/web/packages/factoextra/index. html Pheatmap v1.0.12 CRAN edgeR v3.36.0 162 Trimmomatic v.0.36 156 STAR aligner 157 Subread v.1.5.2 158 159 160 161 163 Burrows-Wheeler Aligner 164 v0.5.9 Picard MarkDuplicates v1.67 165 Model-Based Analysis of ChIP- |[166 seq Bedtools v2.16.2 167 https://cran.r- project.org/web/packages/pheatmap/pheat map.pdf Diffbind v2.14.0 168 MetaCycle v1.2.0 169 32



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